Mushroom culture from Spore

If you purchased a spore print or a culture syringe to start your mushroom cultivation, your next step would be to learn how to prepare AGAR media. Agar is a seaweed extract, a gelatinous substance that will solidify at room temperature. It can be sterilized by pressure cooking and is ideal food for mushroom cultures to thrive on. You will need a sterile space, a glove box or a laminar flow hood to do agar work.


See This Formula

Tinfoil and cotton Cap

Agar to Master Spawn

Once you have a culture growing on agar, transfer it to sterilized sorghum or rye  grains. You can start this by either using a 500ml Glass JAR or a unicorn type bag with filter patch. I prefer the bags, as jars can be a lot of extra work. When using a unicorn bag, fill it only with 1200g or so of seed mix in the bottom of the bag, not too much. The seed mix is pre soaked for 12 hours then dried again as to not feel wet to the touch. Sterilize the bag of grain - refer to sterilization, wait for it to cool, then in a sterile environment or a glove-box, inoculate the seed bag with your shiitake agar wedge. Leave the agar on top of the seed mix, where it drops. Gently close the bags, and place in a clean space, to incubate at 20 - 24 degrees Celsius. Let the mycelium grow out for about a week, and then give it a good shake, to disperse the fine growth of mycelium right throughout the grain bag. Place the bag back to incubate for another week. By now the mycelium would have consumed every last corner of the seed bag, and well on its way to becoming a white mass of master spawn.