Category Archives: Substrate Preparation

Sterilise seed or a wooded substrate

sterilise mushroom substrate

What you will need: a Stove top a Pressure cooker or autoclave glass containers (Console jars) or breathable filtered bags a suitable wooded substrate (for growing mushrooms) or seed (for producing spawn) water The process: Once your wooded substrate or seed has been hydrated to the required level, fill your glass containers or breathable filtered […]

Substrate formula for mushroom Cultivation

mushroom substrate formula

This formula will apply to creating a substrate for cultivating Saprophytic mushrooms. Dry mix together your ingredients first to get an even spread of all the added nutrients and moisture. 10 kg Wood shavings/chips 1 kg Bran 100 gr Gypsum Supplement with (Optional): 100 – 500 gr *Lucerne Powder or Cotton seed waste (for Oyster […]

Preparing Agar (PDA)

PDA Culture Media Dishes

Preparing your Agar For every 1 Litre of Agar Final Mixed Solution you will need the following. 20 grams Bacteriological Agar 200g Potato infusion 4 g of Malt Extract or Yeast Extract (optional) 20 g Dextrose 1 Litre of Distilled Water Autoclave or Pressure Vessel Method: Place your cleaned Erlenmeyer flask on a kitchen scale […]